FIGURE

Fig. 3

ID

ZDB-FIG-121025-27

Publication

Huang et al., 2012 - High-throughput screening for bioactive molecules using primary cell culture of transgenic zebrafish embryos

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

Flk1-GFP-Labeled Endothelial Cells Respond to Growth Factors and Small Molecules in Primary Culture (A–I) Endothelial cell proliferation and differentiation derived from Flk1-GFP are significantly promoted by 100 ng/ml bFGF (C, bright field, and D) compared with control (A, bright field, and B) at 22 hr after culture. Recombinant VEGF121 (20 ng/ml, F), small molecule SB-431542 (2 μM, G) and the combination of both (H) also remarkably enhance Flk1-GFP endothelial tube formation compared with control (E) at day 5, which exhibits a significant increase in total tube length (I-1, control; I-2, 2 μM SB431542; I-3, 20 ng/ml VEGF121; I-4, 2 μM SB431542 and 20 ng/ml VEGF121). The number values represent total tube length. See also Figure S3.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.