Fig. 6

Wnt Signaling Inhibits the Production of Tanycytes from Adult Hes1⁺ Progenitors (A and B) Coronal and sagittal sections of adult Hes1^C2/+; R26R^LacZ/+ mouse brains, 5 days and 9 months after TM administration at P60. (C and D) Coronal cryosections through the hypothalamus of adult Hes1^C2/+; R26R^EYFP/+ mouse brains, 5 days and 9 months after TM administration at P60. (E) The three types of EYFP⁺ cells observed at 2 months post-TM. (F) Expression of EYFP and GFAP in Hes1^C2/+; R26R^EYFP/+ mice 2 months post-TM. (G and H) Marker analysis of Hes1^C2/+; R26R^EYFP/+ mice 9 months post-TM. Boxes in (G) show enlarged ventricular (yellow) and parenchymal (red) zones in which cell types are indicated by arrowheads. All EYFP⁺ cells are Sox2⁺, and some ventricular Type A cells are also GFAP⁺. (H) Expression of EYFP and NeuN⁺ in the parenchymal zone, where no colabeled cells are observed. (I) Percentage of Type A, B, and C EYFP⁺ cells 2 months following β-catenin inactivation or activation. Single confocal optical sections are shown in (C–H). Scale bars represent 1 mm (A and B), 80 μm (C, D, F, G, and H), and 10 μm (E). Cell counts were collected from the mediobasal hypothalamus, using six 40 μm sections from three mice for each genotype. *p < 0.05. Error = ±SD.