FIGURE

Fig. 3

ID

ZDB-FIG-120914-20

Publication

Gupta et al., 2012 - A Splice Site Mutation in Laminin-alpha2 Results in a Severe Muscular Dystrophy and Growth Abnormalities in Zebrafish

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Fig. 3

Laminin-α2 deficiency results in severe muscle degeneration.

(A–B) Wholemount immunofluorescence analysis showed reduced levels of laminin complex in myotendinous junctions in the mutant muscles. (C–F) The expression of β-dystroglycan as well as dystrophin was also reduced in the mutant muscles. (G–J) Myofibers in wild-type muscles are attached to either side of the myotendinous junctions and displayed well-organized muscles. Mutant fish, however, displayed highly disorganized muscles. Several detached myofibers lacking the contractile proteins are seen in the mutant muscles by α-actinin and sarcomeric actin antibody staining (arrows). (K) Evans blue dye (EBD) injections at 3 dpf detected occasional staining in necrotic fibers (arrow). Bars = 10 μm.

Expression Data

Antibodies:	Ab1-actn Ab1-dmd Ab1-lama1 Ab3-dag1
Fish:	AB lama2^cl501/cl501 (AB)
Anatomical Terms:	skeletal muscle cell vertical myoseptum
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	lama2^cl501/cl501 (AB)
Observed In:	muscle
Stage:	Day 5

Phenotype Detail

Acknowledgments

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