FIGURE

Fig. 2

ID

ZDB-FIG-120914-19

Publication

Gupta et al., 2012 - A Splice Site Mutation in Laminin-alpha2 Results in a Severe Muscular Dystrophy and Growth Abnormalities in Zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Abnormal myosepta and myofiber detachment in laminin-α2 deficient lama2^cl501/cl501 fish.

(A & B) Hematoxylin and eosin staining of longitudinal sections of wild-type and lama2 mutant fish at 5 dpf. Mutant muscles showed highly disorganized myofibers in the affected somites with irregular myosepta boundaries (arrowhead) and eosin positive detached myofibers (arrow). (C–D) Cross-sections of wild-type and mutation fish also showed smaller myotome and degenerating muscle fibers in lama2 mutant fish at 5 dpf (arrows), bars = 10 μm. (E & H) Electron microscopy showed myofiber detachment from the myotendinous junction (MTJ) in mutant muscles (arrow). (F & I) The myofibers in wild-type muscles attached tightly to the surrounding fibers while mutant muscle displayed large gaps in the extracellular matrix between adjacent fibers and disorganized Z- lines (black arrow) and M-lines (while arrow). (G & J) Defects in extracellular-matrix results in damaged myofibers in the myotome (cross-section). A large number of apoptotic nuclei were observed in the mutant muscles (J, arrow).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	lama2^cl501/cl501 (AB)
Observed In:	muscle myoseptum myotome myotome muscle tendon junction skeletal muscle skeletal muscle cell M band skeletal muscle cell myofibril skeletal muscle cell Z disc
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One