FIGURE

Fig. 4

ID

ZDB-FIG-120824-27

Publication

Schoenebeck et al., 2012 - Variation of BMP3 Contributes to Dog Breed Skull Diversity

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Fig. 4

Zebrafish cranioskeletal development requires Bmp3 function.

(A–H) Wholemount RNA in situ hybridization of bmp3 expression at 48 hpf (A,B), 72 hpf (C), and 96 hpf (D) stages. Anterior to the left. (A) Dorsal view, (B–D) lateral view. Pharyngeal arches indicated by brackets, pectoral fins by red arrowheads. Wholemounts (E,H,K) and alcian blue cartilage stains (F,G,I,J,L,M) of 96 hpf embryos from uninjected (E–G) and morpholino-injected embryos (h–j, mild phenotype, n = 72/177; k–m, severe phenotype, n = 83/177). Phenotypic severity is distinguished by tail curling (compare insets). Loss of jaw structures (black arrows) and frontal bossing (white arrowheads) is apparent in both classes of morphants. Cartilage is severely dysmorphic, hypoplastic, or absent following Bmp3 knockdown. Abbreviations correspond to ceratobranchial (cb), ceratohyal (ch), eythmoid plate (ep), hyosymplectic (hs), Meckel′s (m), palatoquadrate (pq), and trabeculae (tr) cartilages.

Expression Data

Gene:	bmp3
Fish:	WT
Anatomical Terms:	pectoral fin pharyngeal arch
Stage Range:	Long-pec to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-bmp3
Knockdown Reagent:	MO2-bmp3
Observed In:	cranial cartilage embryonic cranial skeleton morphogenesis neurocranium pharyngeal arch cartilage
Stage:	Day 4

Phenotype Detail

Acknowledgments

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