FIGURE

Fig. 1

ID
ZDB-FIG-120816-6
Publication
Provost et al., 2012 - Ribosomal biogenesis genes play an essential and p53-independent role in zebrafish pancreas development
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Fig. 1

Zebrafish sbds is an essential developmental gene with high-level expression in pancreas and surrounding tissues. (A) Expression of sbds examined by in situ hybridization in wild-type zebrafish embryos at 120 hpf. The boxed pancreatic region is shown at higher magnification to illustrate pancreatic and peri-pancreatic expression (arrowheads) of sbds. (B) Adult zebrafish pancreas (boxed) expresses sbds. (C) SbdsSpl MO results in durable loss of sbds transcript as assessed by RT-PCR. (D) Survival curve for recipients of SbdsATG MO illustrates near complete embryonic lethality by 7 dpf, as compared with mock injected embryos. (E) Ribosome profile of lysate prepared from 48-hpf control and SbdsATG MO-injected embryos. (F) Quantification of ribosomal subunit ratios, monosomes and polysomes in control and SbdsATG MO-injected embryos. Error bars indicate mean ± s.e.m. i, intestine; L, liver; P, pancreas.

Expression Data
Gene:
Fish:
Anatomical Term:
Stage Range: Day 5 to Adult

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Long-pec to Days 7-13

Phenotype Detail
Acknowledgments
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