FIGURE

Fig. 3

ID

ZDB-FIG-120601-41

Publication

Choe et al., 2012 - A Gal4/UAS system for conditional transgene expression in rhombomere 4 of the zebrafish hindbrain

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Fig. 3

Transgenic embryos from the hoxb1a(β-globin):Gal4VP16^um60 line are indistinguishable from their non-transgenic siblings with respect to expression of all hindbrain genes tested. Embryos were collected at the indicated stages and assayed for expression of hoxb1a (A–C), Gal4 (D, F, H), valentino (E), krox20 (F; detected in red), or hoxa3a (G) by in situ hybridization. I: hoxb1a(β-globin):Gal4VP16^um60 embryos were injected with a 10× UAS:GFP plasmid and assayed for GFP fluorescence at 24hpf. All embryos are from an outcross of a heterozygous hoxb1a(β-globin):Gal4VP16^um60 carrier. Embryos in D, F, H, I are heterozygous for the hoxb1a(β-globin):Gal4VP16^um60 transgene while embryos in A–C, E, G may be either heterozygous or non-transgenic, but there is no difference in expression of hoxb1a, valentine, hoxa3a, or krox20 within the clutch of embryos. Arrows in H and I indicate expression in r4. A–G are dorsal views with anterior to the top (A, B) or the left (C–G). H and I are in lateral view with anterior to the left.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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