Fig. 3

Universal knockdown of ctnnb1 and ctnnb2 disturbs spaw and Nodal-regulated gene expression and impairs the midline barrier establishment. One-cell embryos were injected with indicated morpholinos. (A-D2) spaw expression in the perinodal region (black arrows) and left LPM (red arrows) at the six-somite (A-D) and 13-somite (A2-D2) stages, dorsal views. Embryos were staged by counting somite numbers. In the bottom left-hand corner was the number of embryos with the representative spaw expression around KV/total number of embryos. (E-L) Representative images of spaw expression in LPM at the 23-somite stage. (M,N) Statistical data for spaw in the LPM, lft1 in the diencephalon and lft2 in the heart primordium at 21.5 hpf (M) and 23 hpf (N), respectively. n, observed embryo number. (O-X) The representative patterns of ntl and lft1 in the midline (notochord) at about the 19- and 16-somite stages, respectively. The ratios are indicated in the bottom left-hand corner. Scale bars: 100 μm.