FIGURE

Fig. S1

ID

ZDB-FIG-120427-17

Publication

Singh et al., 2012 - Regeneration of amputated zebrafish fin rays from de novo osteoblasts

Other Figures

All Figure Page

Back to All Figure Page

Fig. S1

Osteoblast-Specific Transgenic Reporter and Lineage-Labeling Strains

(A-D) Transverse section through an uninjured caudal fin from an osx:mCherry; osc:EGFP double transgenic animal. Fin hemirays are outlined by osx:mCherry⁺ osteoblasts, most of which are also positive for osc:EGFP fluorescence. (E-H) Regenerating fins of osx:mCherry; osc:EGFP animals shown at 4 (E, F) and 8 (G, H) dpa. osx:mCherry is easily visible in the regenerate by 4 dpa, while osc:EGFP fluorescence emerges at 7-8 dpa. Arrowheads indicate amputation planes. (I) Bright field (left) and fluorescent (right) images of a 6 dpf osx:CreER larva, indicating blue mTagBFP fluorescence in bone structures. Asterisk, yolk autofluorescence. cl, clethium; op, opercule. (J) Section through a 4 dpa β-act2:RSG fin regenerate. DsRed fluorescence is detectable in most cells, indicating that the line is suitable for genetic fate-mapping of fin tissues.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Cell, 22(4), Singh, S.P., Holdway, J.E., and Poss, K.D., Regeneration of amputated zebrafish fin rays from de novo osteoblasts, 879-886, Copyright (2012) with permission from Elsevier. Full text @ Dev. Cell