ZFIN ID: ZDB-FIG-120405-88
Wang et al., 2012 - LAR Receptor Tyrosine Phosphatases and HSPGs Guide Peripheral Sensory Axons to the Skin. Current biology : CB   22(5):373-382 Full text @ Curr. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. 2

Knocking Down ptprfa and ptprfb Caused Skin Innervation Defects (A) Schematic showing the relationship between skin and axons. (B–C) Confocal projections of lateral views of WT (B) and ptprfa/ptprfb double morphant (C) embryos. 90° rotations of the images in (B) and (C) provide cross-section views of these embryos (B2–C2). Zebrafish larvae carried two transgenic reporters—sensory:GFP to visualize somatosensory neurons and krt4:dsRed to visualize the skin. Arrowhead indicates axons innervating internal tissues. Scale bar represents 100 μm. (D) Frequency of embryos with axons below the skin in a defined region of the trunk. Knocking down ptprfa or ptprfb alone caused mild skin innervation defects compared with embryos injected with an equivalent amount of control morpholino (9.7%). However, axons grew below the skin in 78.6% of ptprfa-MO1/ptprfb-MO1 double morphants and 59.2% of ptprfa-MO2/ptprfb-MO2 double morphants, which was significantly (p < 0.001) more than controls. Data was analyzed with two-sided Fisher′s exact test and computed with R (http://www.r-project.org). See Supplemental Information for details of statistical analysis. Con, control; MO, morphants; ***p < 0.001. See also Figure S2 and mmc2VIDEO and mmc3VIDEO.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed la203Tg; zf154Tg standard conditions Long-pec integument IFL
EGFP la203Tg; zf154Tg standard conditions Long-pec Rohon-Beard neuron IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
la203Tg; zf154Tg + MO1-ptprfa + MO1-ptprfb standard conditions Long-pec Rohon-Beard neuron axon mislocalized adaxially, abnormal
Long-pec Rohon-Beard neuron axon subdermal, abnormal
Long-pec sensory neuron axon guidance disrupted, abnormal
la203Tg; zf154Tg + MO1-ptprfa + MO2-ptprfa standard conditions Long-pec Rohon-Beard neuron axon mislocalized adaxially, abnormal
Long-pec Rohon-Beard neuron axon subdermal, abnormal
Long-pec sensory neuron axon guidance disrupted, abnormal
la203Tg; zf154Tg + MO2-ptprfa + MO2-ptprfb standard conditions Long-pec Rohon-Beard neuron axon mislocalized adaxially, abnormal
Long-pec Rohon-Beard neuron axon subdermal, abnormal
Long-pec sensory neuron axon guidance disrupted, abnormal
la203Tg; zf154Tg + MO2-ptprfb standard conditions Long-pec Rohon-Beard neuron axon mislocalized adaxially, abnormal
Long-pec Rohon-Beard neuron axon subdermal, abnormal
Long-pec sensory neuron axon guidance disrupted, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Curr. Biol.