FIGURE

Fig. 1

ID

ZDB-FIG-120315-65

Publication

Schaub et al., 2012 - Mutation of zebrafish Snapc4 is associated with loss of the intrahepatic biliary network

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Fig. 1

Morphological and physiological phenotypes of s445 mutant larvae at 120 hpf. (A and B) Bright-field images of wild-type (A) and s445 mutant (B) larvae at 120 hpf are overlaid with Tg(gutGFP)^s854 expression (green). The physiological appearance of s445 mutant larvae is normal, however their liver size is smaller at 120 hpf. Lateral views, anterior to the left. White arrowheads point to the liver. (C and D) Fluorescent image of wild-type (C) and s445 mutant (D) Tg(fabp1a:DsRed)^gz15 larvae at 120 hpf. Liver size visualized by Tg(fabp1a:DsRed)^gz15 expression is smaller in s445 mutant larvae. Lateral views, anterior to the left. (E and F) Fluorescent images of wild-type (E) and s445 mutant (F) larvae soaked in PED6. PED6 is processed by phospholipase and fluorescence is detected in the intestine in both wild-type and s445 mutant larvae. However, PED6 fluorescence is missing from the s445 mutant gallbladder. Lateral views, anterior to the right. Asterisks indicate the intestine. (G and H) Projected confocal image of the gallbladders of wild-type (G) and s445 mutant (H) Tg(gutGFP)^s854 larvae. The gallbladder and the extrahepatic bile duct visualized by Tg(gutGFP)^s854 expression are schematically presented in (G2 and H2). Ventral views, anterior to the top.

Expression Data

Genes:	DsRed GFP
Fish:	gz15Tg s854Tg snapc4^s445/s445; gz15Tg snapc4^s445/s445; s854Tg
Anatomical Terms:	extrahepatic duct gall bladder gut liver
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	snapc4^s445/s445; gz15Tg snapc4^s445/s445; s854Tg
Observed In:	liver liver and biliary system
Stage:	Day 5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 363(1), Schaub, M., Nussbaum, J., Verkade, H., Ober, E.A., Stainier, D.Y., and Sakaguchi, T.F., Mutation of zebrafish Snapc4 is associated with loss of the intrahepatic biliary network, 128-137, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.