FIGURE

Fig. 9

ID

ZDB-FIG-120301-5

Publication

van Eeden et al., 1996 - Mutations affecting somite formation and patterning in the zebrafish, Danio rerio

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Fig. 9

(A) Engrailed (4D9) staining of a 21-somite stage wild-type embryo. Staining is visible at the midbrain-hindbrain boundary and in the somites. In every somite about 2-5 nuclei, representing the muscle pioneers, are stained. In a yot mutant at the same stage (B), staining of the muscle pioneers is absent. (C) 4D9 staining of a wildtype sibling and (D) the weakest you-type mutant, you^tm146c. Approximately one Engrailed-positive cell is present per segment. (E-G) myoD-staining of a wild type, a yot mutant and a putative yot/+ embryo at the 17-somite stage, respectively. In the homozygous yot mutant, adaxial staining is absent and the staining in the rest of the paraxial mesoderm is reduced (G). In the yot heterozygous embryo, expression in the adaxial cells is reduced, especially in the presomitic mesoderm, anterior to the tailbud (F).

Expression Data

Gene:	myod1
Antibody:	Ab1-eng
Fish:	WT gli2a^ty119/ty119 scube2^{tm146c/tm146c} gli2a^ty119/+
Anatomical Terms:	adaxial cell midbrain hindbrain boundary muscle pioneer nucleus somite muscle pioneer
Stage Range:	14-19 somites to 20-25 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gli2a^ty119/ty119 scube2^{tm146c/tm146c} gli2a^ty119/+
Observed In:	adaxial cell muscle pioneer somite
Stage Range:	14-19 somites to 20-25 somites

Phenotype Detail

Acknowledgments

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