Fig. 6
- ID
- ZDB-FIG-120216-36
- Publication
- McKeown et al., 2012 - Disruption of Eaat2b, a glutamate transporter, results in abnormal motor behaviors in developing zebrafish
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slc1a2b mRNA is expressed in glial cells at 48 hpf. (A) The 3A10 antibody labels the Mauthner cell in the hindbrain. The scale bar = 50 μm. (B) slc1a2b mRNA is expressed in the hindbrain in cells around the Mauthner cell. (C) The merged images show that slc1a2b mRNA is not detected within the Mauthner cell. (D–F) Higher magnification views of a Mauthner cell, slc1a2b staining, and the merged images. The scale bar in this and all following panels = 10 μm. (G–I) Lateral views of Tg(mnx1:GFP) larvae, which express GFP in motor neurons and some ventral interneurons, stained for slc1a2b mRNA expression. Little colocalization between GFP+ neurons and slc1a2b mRNA was observed. (J–K) Cross-sectional views of the spinal cord of Tg(gfap:GFP) transgenic larvae. GFAP is expressed predominantly in glial cells at this stage of development. slc1a2b expression was detected in some GFAP-positive cells. (M–O) Lateral views of Tg(gfap:GFP) larvae stained for slc1a2b mRNA expression. The arrowheads in J indicate the plane in which the lateral views are taken. The arrows indicate examples of a GFP+ cell in which slc1a2b expression was detected. Similar results were obtained using 96 hpf larvae. |
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| Stage: | Long-pec |
Reprinted from Developmental Biology, 362(2), McKeown, K.A., Moreno, R., Hall, V.L., Ribera, A.B., and Downes, G.B., Disruption of Eaat2b, a glutamate transporter, results in abnormal motor behaviors in developing zebrafish, 162-171, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.