FIGURE

Fig. 10

ID

ZDB-FIG-120201-9

Publication

Li et al., 2011 - Smyd1b_tv1, a Key Regulator of Sarcomere Assembly, Is Localized on the M-Line of Skeletal Muscle Fibers

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Fig. 10

Knockdown of endogenous Smyd1b advances the timing of sarcomeric localization of Smyd1b_tv1^myc in zebrafish embryos.

A. Smyd1b E9I9-MO was injected into Smyd1b_tv1^myc or Smyd1b_tv1^myc transgenic zebrafish embryos at 1?2 cell stages. Western blot analysis shows the expression of myc-tagged Smyd1b_tv1^myc and Smyd1b_tv1^myc in un-injected control or E9I9-MO injected transgenic zebrafish embryos at 24 hpf. γ-Tubulin was used as loading control. B and C. Immunostaining using anti-myc antibody shows the cytoplasmic (B) or sarcomeric localization (C) of smyd1b_tv1^myc in control (B) or E9I9-MO injected (C) transgenic zebrafish embryos at 24 hpf. D and E. Immunostaining using anti-myc antibody shows the cytoplasmic localization of smyd1b_tv2^myc in control or E9I9-MO injected transgenic zebrafish embryos at 24 hpf. Scale bar: 30 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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