FIGURE

Fig. 11

ID

ZDB-FIG-120201-10

Publication

Li et al., 2011 - Smyd1b_tv1, a Key Regulator of Sarcomere Assembly, Is Localized on the M-Line of Skeletal Muscle Fibers

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Fig. 11

The effect of hsp90α1 mutation or knockdown on Smyd1b_tv1 sarcomeric localization.

A and D. Immunostaining using anti-myomesin antibody shows the organization of myomesin in hsp90α1 mutant (A), or control (D) smyd1b_tv1^myc transgenic embryos at 72 hpf. B and E. Immunostaining using anti-myc antibody shows the localization of Smyd1b_tv1^myc in hsp90α1 mutant (B), or control (E) smyd1b_tv1^myc transgenic embryos at 72 hpf. C and F. F59 staining shows the organization of slow muscle myosin in hsp90α1 mutant (C), or control (F) smyd1b_tv1^myc transgenic embryos at 72 hpf. G and H. DNA construct Smyd1_tv1-EGFP was injected alone or together with hsp90α1 ATG-MO into zebrafish embryos. Smyd1_tv1-EGFP localization was determined in myofibers of the control (G), or hsp90α1 knockdown (H) at 48 hpf. Scale bars: D = 40 μm; G = 15 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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