ZFIN ID: ZDB-FIG-111220-24
Cheung et al., 2012 - Regulation of intrahepatic biliary duct morphogenesis by Claudin 15-like b. Developmental Biology   361(1):68-78 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 5

Fig. 3 Cldn15lb is dynamically expressed in hepatocytes and biliary epithelial cells during development. a–b, Single confocal plane of MDCK cysts stably expressing Cldn15lb-GFP immunostained with the tight junction markers ZO-1(a) or Occludin (b). Cldn15lb-GFP co-localizes with the tight junction markers (arrows). c–e, 150 μm transverse sections through the liver at 80 (c), 100 (d), and 120 (e) hpf. c, At 80 hpf, Cldn15lb immunostaining is observed in biliary epithelial cells (BECs) (arrows) as marked by Tg(Tp1bglob:eGFP) expression as well as the surrounding hepatocytes (arrowheads). d, At 100 hpf, Cldn15lb immunostaining in BECs (arrows) increases while that in hepatocytes (arrowheads) becomes more punctate. e, At 120 hpf, Cldn15lb immunostaining is only observed in BECs (arrows). Green: Tg(Tp1bglob:eGFP); red: Cldn15lb antibody. The antibody also labels cells in the gut (g). Scale bars are 10 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cldn15lb Ab1-cldn15lb um14Tg standard conditions Protruding-mouth gut epithelium IHC
Ab1-cldn15lb Protruding-mouth hepatic duct epithelial cell IHC
Ab1-cldn15lb Protruding-mouth hepatocyte IHC
Ab1-cldn15lb Day 4 gut epithelium IHC
Ab1-cldn15lb Day 4 hepatic duct epithelial cell IHC
Ab1-cldn15lb Day 4 hepatocyte IHC
Ab1-cldn15lb Day 5 gut epithelium IHC
Ab1-cldn15lb Day 5 hepatic duct epithelial cell IHC
EGFP um14Tg standard conditions Protruding-mouth hepatic duct epithelial cell IFL
Day 4 hepatic duct epithelial cell IFL
Day 5 hepatic duct epithelial cell IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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Reprinted from Developmental Biology, 361(1), Cheung, I.D., Bagnat, M., Ma, T.P., Datta, A., Evason, K., Moore, J.C., Lawson, N.D., Mostov, K.E., Moens, C.B., and Stainier, D.Y., Regulation of intrahepatic biliary duct morphogenesis by Claudin 15-like b, 68-78, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.