ZFIN Image: Cheung et al., 2012, Fig. 3

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Figure Caption

Fig. 3 Cldn15lb is dynamically expressed in hepatocytes and biliary epithelial cells during development. a–b, Single confocal plane of MDCK cysts stably expressing Cldn15lb-GFP immunostained with the tight junction markers ZO-1(a) or Occludin (b). Cldn15lb-GFP co-localizes with the tight junction markers (arrows). c–e, 150 μm transverse sections through the liver at 80 (c), 100 (d), and 120 (e) hpf. c, At 80 hpf, Cldn15lb immunostaining is observed in biliary epithelial cells (BECs) (arrows) as marked by Tg(Tp1bglob:eGFP) expression as well as the surrounding hepatocytes (arrowheads). d, At 100 hpf, Cldn15lb immunostaining in BECs (arrows) increases while that in hepatocytes (arrowheads) becomes more punctate. e, At 120 hpf, Cldn15lb immunostaining is only observed in BECs (arrows). Green: Tg(Tp1bglob:eGFP); red: Cldn15lb antibody. The antibody also labels cells in the gut (g). Scale bars are 10 μm.

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Expression / Labeling details

Acknowledgments

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Reprinted from Developmental Biology, 361(1), Cheung, I.D., Bagnat, M., Ma, T.P., Datta, A., Evason, K., Moore, J.C., Lawson, N.D., Mostov, K.E., Moens, C.B., and Stainier, D.Y., Regulation of intrahepatic biliary duct morphogenesis by Claudin 15-like b, 68-78, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.