FIGURE

Fig. 4

ID

ZDB-FIG-111220-2

Publication

Akten et al., 2011 - Interaction of survival of motor neuron (SMN) and HuD proteins with mRNA cpg15 rescues motor neuron axonal deficits

Other Figures

Fig. 4

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Fig. 4

Cpg15 partially rescues SMN deficiency. (A-C) Lateral-view representative images of Tg(hb9:GFP) embryos at 28 hpf uninjected (A), injected with 9 ng of smn MO (B), and injected with 9 ng of smn MO and 200 ng of full-length human cpg15 mRNA (C). (D) Full-length human cpg15 rescues motor axon defects caused by a reduction of Smn in zebrafish. Tg(hb9:GFP) zebrafish injected with 9 ng of smn MO and scored at 28 hpf using previously published criteria (29) resulted in a distribution of fish with motor nerve defects (n = 260 fish, 5,200 nerves, three injections). Coinjection of 9 ng of smn MO with 200 pg of full-length human cpg15 mRNA was able to partially rescue the nerve defects (n = 326 fish, 6,520 nerves; P < 0.01). The distribution of larval classifications (severe, moderate, mild, and no defects) was analyzed using the Mann–Whitney nonparametric rank test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ml2Tg + MO1-smn1 + MO2-smn1
Knockdown Reagents:	MO1-smn1 MO2-smn1
Observed In:	motor neuron axon
Stage:	Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA