FIGURE

Fig. 4

ID

ZDB-FIG-111202-29

Publication

Singh et al., 2011 - Common DISC1 Polymorphisms Disrupt Wnt/GSK3β Signaling and Brain Development

Other Figures

Fig. 4

All Figure Page

Back to All Figure Page

Fig. 4

The DISC1 R264Q and L607F Variants Are Unable to Rescue Abnormal Brain Development in Zebrafish Due to DISC1 Loss of Function

Embryos were injected with Control MO or Disc1 MO, together with mRNA encoding DISC1 variant proteins or GFP, and assayed at 30 hpf in three independent experiments. Morpholino (3.5 ng) and mRNA (200 pg) were injected/embryo.

(Ai–Fi) Lateral images of the whole embryo.

(Bii–Fii) images of the dorsal view of the head.

(Aiii–Fiii) Somites stained for phalloidin (actin filaments).

(Aiv,v–Fiv,v) Neurons and axon tracts stained for acetylated tubulin.

ac, anterior commissure; poc, postoptic commissure; sot, supraoptic tract. z, Quantification of the percentage of embryos displaying a curved tail in each condition that is similar to DISC1-dependent Wnt signaling. ^**p < 0.005, ns = not significant. Error bars represent mean ± SEM. All scale bars represent 50μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO5-disc1
Knockdown Reagent:	MO5-disc1
Observed In:	brain forebrain muscle post-vent region ventricular system
Stage:	Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Neuron