FIGURE

Fig. 9

ID

ZDB-FIG-111128-42

Publication

Gallagher et al., 2011 - Rbfox-regulated alternative splicing is critical for zebrafish cardiac and skeletal muscle functions

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Fig. 9

Myofibril defects are rescued with exogenous rbfox2. (A–C) Anti-MHC immunohistochemistry at 48 hpf reveals that slow muscle fiber thick filament morphology in rbfox2 mRNA-injected double morphant embryos closely resembles wild-type morphology and is substantially improved compared to that in embryos injected with rbfox1l/rbfox2 MOs alone, although some wavy fibers persist. (D–I) Similarly, thin filament structure detected using Alexa-Fluor 488-conjugated phalloidin appears elongated and highly organized in both deeper fast muscle and more superficial slow muscle layers of rbfox2 mRNA-injected double morphants when compared to mock-injected wildtype controls and embryos injected with rbfox1l/rbfox2 MOs alone. Scale bar = 50 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 359(2), Gallagher, T.L., Arribere, J.A., Geurts, P.A., Exner, C.R., McDonald, K.L., Dill, K.K., Marr, H.L., Adkar, S.S., Garnett, A.T., Amacher, S.L., and Conboy, J.G., Rbfox-regulated alternative splicing is critical for zebrafish cardiac and skeletal muscle functions, 251-61, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.