Fig. 3
Following Pax7a+ve cell division in live embryos. A: Sequences of images of a 34 hours post fertilization (hpf). TgBAC(pax7a:GFP)i131 embryo captured every 90 sec. Individual GFP+ve cells (red arrows) can be seen dividing in each somite. A–G, A2–D2, A3–G3: Three different cell divisions are shown. In each case, one of the two daughter cells moves into the myotome while the other remains close to the vertical myoseptum. B: Series of images of a TgBAC(pax7a:GFP)i131 embryo at 58 hpf showing a GFP+ve cell (red arrow) dividing in the dorsal somite (A*–L*). The more ventral daughter cell appears to elongate to form a myofiber. The individual images were taken approximately 160 sec apart. Scale bar = 50 μm. |