Fig. 2

PRMT5 and CARM1 morphants exhibit distinct and specific phenotypes.

(A, B) One-cell embryos were injected with 6 ng of either a control morpholino (MoCt), or a morpholino against PRMT5 (MoP5) or CARM1 (MoC1). Embryos were collected at 14 ss and were processed for immunoblotting to detect (A) PRMT5 and (B) CARM1 expression. Tubulin was used as a loading control. (C) Lateral (anterior to left) and dorsal view (anterior to top) of 14–16 ss zebrafish embryos injected with the indicated morpholino. Rescue experiments by injection of the cognate mRNA are shown to the right (MoP5+P5, MoC1+C1). Schematic representations of each somite are shown below the corresponding dorsal view. Mo, morpholino. (D) Phenotypes of 24 hpf embryos injected with increasing doses (3-6-12 ng) of Control (MoC, a–c), PRMT5 (MoP5, d–f) or CARM1 (MoC1, g–i) morpholinos or 6 ng of morpholino against PRMT5 or CARM1 co-injected with their corresponding mRNA (MoP5+P5, j and MoC1+C1, k) for rescue experiment. Embryos were visualized at 24 hpf. Lateral view, anterior to the left.