FIGURE

Fig. 3

ID

ZDB-FIG-111018-4

Publication

Patra et al., 2011 - Nephronectin regulates atrioventricular canal differentiation via Bmp4-Has2 signaling in zebrafish

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Fig. 3

Npnt knockdown causes extension of the AV canal. (A-D) Projections of confocal images of hearts from control (A,C) and MO2-injected (B,D) embryos from transgenic Tg(myl7:EGFP-HsHRAS)^s883 (A,B) and Tg(?5.1myl7:nDsRed2)f2 (C,D) zebrafish at 52 hpf, suggesting an extension of the AV canal (brackets) in npnt morphants. (E) Representative images of hearts from control, MO2-injected and MO2 + npnt mRNA-injected embryos from transgenic Tg(myl7:EGFP-HsHRAS)^s883 zebrafish at 52 hpf. Brackets indicate the AV boundary. (F) Quantitative analysis (n>160 from three independent experiments; mean � s.e.m.). Note that injection of npnt mRNA rescued the MO2-mediated AV canal extension. (G) Quantitative analysis scoring of type I (mild extension of the AV canal), type II (obvious extension of the AV canal) and type III (straight heart) AV canal defects (mean � s.e.m.). A, atrium; V, ventricle. Scale bars: 50 μm.

Expression Data

Genes:	DsRed2 EGFP
Fish:	f2Tg s883Tg f2Tg + MO2-npnta s883Tg + MO2-npnta
Knockdown Reagent:	MO2-npnta
Anatomical Terms:	cardiac muscle cell nucleus cardiac muscle cell plasma membrane
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	f2Tg + MO2-npnta s883Tg + MO2-npnta
Knockdown Reagent:	MO2-npnta
Observed In:	atrioventricular canal atrioventricular valve heart
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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