FIGURE

Fig. 5

ID

ZDB-FIG-111018-28

Publication

Zhu et al., 2011 - Evaluation and application of modularly assembled zinc-finger nucleases in zebrafish

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Fig. 5

The ZFN-induced um27 lesion is a truncation allele of gata2a. (A) Location of frameshift caused by the um27 deletion in the zebrafish gata2a gene. ZF, zinc-finger domain. (B) gata2a coding and amino acid sequence in the region of the um27 deletion. ZFN recognition sequences are boxed. (C) Genotype of embryos derived from an incross of gata2a^um27 heterozygous carriers. (D) Genotype of embryos with normal circulation derived from a gata2a^um27 incross injected with 200 pg gata2a mRNA. Asterisks denote mutant embryos with normal trunk circulation. (E) Percentage of gata2a^um27 mutant embryos displaying the indicated circulation phenotypes.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gata2a^um27/um27
Observed In:	blood circulation
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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