ZFIN ID: ZDB-FIG-110804-16
Kapsimali et al., 2011 - Fgf signaling controls pharyngeal taste bud formation through miR-200 and Delta-Notch activity. Development (Cambridge, England)   138(16):3473-3484 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Condition:
Anatomical Term:
Stage: Pec-fin
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Pec-fin

Fig. 7

Activation of Notch signaling maintains taste bud Calb2b+ but not 5HT+ cells. (A) Heatshock treatment in HsNicd siblings. (B-G) Embryonic stage and scale bar are in the bottom left-hand corner and marker expression is in the bottom right-hand corner. Anterior is towards the left. Ventral views of whole-mounted larvae heads. Asterisks indicate taste buds in the lips. Note the absence of 5HT (C) and maintenance of Calb2b (E) and miR-200b (G) expression in the Hsnicd-expressing embryos compared with wild-type (heatshocked) siblings. Hsnicd embryos were identified by anti-myc (red, C,E) immunohistochemistry or genotyped after in situ hybridization (G). Scale bars: 12 μm. b1-b5, branchial arches 1-5; e, eye; h, hyoid arch; m, mandibular arch; nm, neuromast; oe, olfactory epithelium.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
calb2b Ab1-calb2 WT standard conditions Pec-fin pharyngeal arch taste bud IHC
Ab1-calb2 kca3Tg/kca3Tg; kca4Tg/kca4Tg heat shock Pec-fin pharyngeal arch taste bud IHC
mir200b WT standard conditions Pec-fin pharyngeal arch taste bud ISH
kca3Tg/kca3Tg; kca4Tg/kca4Tg heat shock Pec-fin pharyngeal arch taste bud ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-serotonin IHC WT standard conditions Pec-fin pharyngeal arch taste bud
IHC kca3Tg/kca3Tg; kca4Tg/kca4Tg heat shock Pec-fin Not Detected pharyngeal arch taste bud
Phenotype Details
Fish Conditions Stage Phenotype
kca3Tg/kca3Tg; kca4Tg/kca4Tg heat shock Pec-fin Notch signaling pathway decreased process quality, abnormal
Pec-fin taste bud formation decreased process quality, abnormal
Pec-fin taste bud physical quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development