ZFIN ID: ZDB-FIG-110804-16 |
Genes: | |
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Antibodies: | |
Fish: | |
Condition: | |
Anatomical Term: | |
Stage: | Pec-fin |
Fish: | |
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Condition: | |
Observed In: | |
Stage: | Pec-fin |
Fig. 7 Activation of Notch signaling maintains taste bud Calb2b+ but not 5HT+ cells. (A) Heatshock treatment in HsNicd siblings. (B-G) Embryonic stage and scale bar are in the bottom left-hand corner and marker expression is in the bottom right-hand corner. Anterior is towards the left. Ventral views of whole-mounted larvae heads. Asterisks indicate taste buds in the lips. Note the absence of 5HT (C) and maintenance of Calb2b (E) and miR-200b (G) expression in the Hsnicd-expressing embryos compared with wild-type (heatshocked) siblings. Hsnicd embryos were identified by anti-myc (red, C,E) immunohistochemistry or genotyped after in situ hybridization (G). Scale bars: 12 μm. b1-b5, branchial arches 1-5; e, eye; h, hyoid arch; m, mandibular arch; nm, neuromast; oe, olfactory epithelium. |
Gene | Antibody | Fish | Conditions | Stage | Anatomy | Assay |
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calb2b | Ab1-calb2 | WT | standard conditions | Pec-fin | pharyngeal arch taste bud | IHC |
Ab1-calb2 | kca3Tg/kca3Tg; kca4Tg/kca4Tg | heat shock | Pec-fin | pharyngeal arch taste bud | IHC | |
mir200b | WT | standard conditions | Pec-fin | pharyngeal arch taste bud | ISH | |
kca3Tg/kca3Tg; kca4Tg/kca4Tg | heat shock | Pec-fin | pharyngeal arch taste bud | ISH |
Antibody | Assay | Fish | Conditions | Stage | Qualifier | Anatomy |
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Ab1-serotonin | IHC | WT | standard conditions | Pec-fin | pharyngeal arch taste bud | |
IHC | kca3Tg/kca3Tg; kca4Tg/kca4Tg | heat shock | Pec-fin | Not Detected | pharyngeal arch taste bud |
Acknowledgments: | ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development |
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