Fig. 5

Fgf is working via nkx2.5 and lycat during gastrulation to establish cardiac and haemagioblast fates. (A) Quantitative PCR on cDNA obtained from RNA extracted from 50% epiboly zebrafish embryos treated with DMSO and SU5402, assayed for lycat and nkx2.5 expression normalised to gapdh. Shown are fold changes in expression, relative to the DMSO control, from three biological replicates. Error bars indicate s.e.m., asterisks indicate statistically significant differences (P<0.001) compared with DMSO controls. (B,C) The scl and etsrp morpholino combination increased amhc and vmhc expression, as seen by in situ hybridisation (B) and qPCR (C; P<0.02) at 48 hpf. However, expression was substantially more upregulated in scl+etsrpMO and nkx2.5 RNA embryos (P<0.05). A similar trend was observed for cmlc2 (P<0.05). Three biological replicates were analysed. Error bars indicate s.e.m., asterisks indicate statistically significant differences compared with wild type and scl+etsrpMO siblings. Scale bars: 100 μm.