FIGURE

Fig. 6

ID

ZDB-FIG-110719-19

Publication

Bajoghli et al., 2004 - An artificial promoter construct for heat-inducible misexpression during fish embryogenesis

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

Transient misexpression with heat stress-inducible constructs. Medaka embryos were co-injected at the one-cell stage with gfp:HSE:luc (10 ng/µl) and meganuclease enzyme. Heat treatment was applied after 24 h at embryonic stage 19 (39°C/2 h). Gfp expression was recorded 5 h (A), 24 h (B), and 72 h (C) after induction. Uninduced embryos were grown until hatching and did not show any gfp expression (D). Yellow staining originates from autofluorescing cells. These larvae were induced (39°C/1 h) and exhibited a strong response after 24 h (E). For comparison, embryos were injected the same way with the HSP70:gfp construct and induced under the same conditions (F, F2, and G). Gfp signals were preferentially observed in yolk cells (F2), gfp-positive cells within the embryo are marked by arrows. (G) The same embryo 48 h later. F is a brightfield view of F2. Abbreviations: od, oil droplet; ey, eye.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 271(2), Bajoghli, B., Aghaallaei, N., Heimbucher, T., and Czerny, T., An artificial promoter construct for heat-inducible misexpression during fish embryogenesis, 416-430, Copyright (2004) with permission from Elsevier. Full text @ Dev. Biol.