Fig. 6

Transient misexpression with heat stress-inducible constructs. Medaka embryos were co-injected at the one-cell stage with gfp:HSE:luc (10 ng/µl) and meganuclease enzyme. Heat treatment was applied after 24 h at embryonic stage 19 (39°C/2 h). Gfp expression was recorded 5 h (A), 24 h (B), and 72 h (C) after induction. Uninduced embryos were grown until hatching and did not show any gfp expression (D). Yellow staining originates from autofluorescing cells. These larvae were induced (39°C/1 h) and exhibited a strong response after 24 h (E). For comparison, embryos were injected the same way with the HSP70:gfp construct and induced under the same conditions (F, F2, and G). Gfp signals were preferentially observed in yolk cells (F2), gfp-positive cells within the embryo are marked by arrows. (G) The same embryo 48 h later. F is a brightfield view of F2. Abbreviations: od, oil droplet; ey, eye.