Distinct alterations in Ca2+ handling induced by altered TNNT2 splicing. Diastolic (A) and transient amplitude (B) Ca2+ measurements in specific heart regions in controls, TNNT2sp morphants and TNNTatg (null) morphants. (C) CTD50 measured in atrium, atrioventricular canal (AVC), outer ventricular curvature (OC), mid-ventricle (MV) and inner ventricular curvature (IC). Asterisk donates P<0.05 for comparison of TNNT2sp morphant with control sample, or TNNT2atg morphant with control. All data expressed as mean ± s.e.m.