FIGURE

Fig. 5

ID

ZDB-FIG-110712-81

Publication

Wiszniak et al., 2011 - HuB (elavl2) mRNA Is Restricted to the Germ Cells by Post-Transcriptional Mechanisms including Stabilisation of the Message by DAZL

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Fig. 5

DAZL protein is able to stabilise the HuB mRNA.

(A) Expression of EGFP and mCherry protein in embryos injected with 150 pg each of EGFP and mCherry-B reporter RNAs, either alone (labelled B), or with ubiquitous overexpression of HA-tagged DAZL, DND, HuB (400 amol RNA) or VASA (200 amol RNA) as labelled. All EGFP images were taken at 2000 ms and all mCherry images were taken at 500 ms. The fold change in the somatic expression of mCherry from the B reporter-protein combination compared to the B reporter alone is indicated as an inset number in white. (B) mCherry-BΔs91011 reporter RNA +/- overexpression of DAZL. (C) Whole-mount in situ hybridisation using an antisense probe directed against the mCherry coding sequence. Embryos at 9 hpf are shown in the left panels (sagittal view with dorsal side to the right), and embryos at 24 hpf are shown in the right panels with germ cells shown as insets.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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