FIGURE

Fig. 4

ID

ZDB-FIG-110706-13

Publication

Chou et al., 2011 - Fascin 2b Is a Component of Stereocilia that Lengthens Actin-Based Protrusions

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

Subcellular localization patterns of wild-type or phosphomutant fascin 2b proteins in live COS-7 cells that coexpress espin.

A cell (A–D) coexpressing GFP-WT fascin 2b and mCherry (mCh)-espin displays colocalization of these proteins in filopodia, which are detected as yellow in image overlays (C,D). Images of cells that coexpress mCherry-espin and either GFP-S38D fascin 2b (E–H) or GFP-S38E fascin 2b (I–L) reveal espin-laden protrusions (red) that lack the fascin 2b phosphomutant proteins (G,H,K,L). Micrographs of a cell that expresses both mCherry-espin and GFP-S38A fascin 2b (M–P) show that both proteins reside in filopodia (yellow) (O,P). Scale bars are 5 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One