FIGURE

Fig. 4

ID

ZDB-FIG-110622-117

Publication

Inoue et al., 2011 - One for all-a highly efficient and versatile method for fluorescent immunostaining in fish embryos

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Fig. 4

Application of heating method to co-staining with fluorescent proteins (FPs) in transgenic lines. (A) PCNA whole-mount immunostaining with Ath5::GFP medaka transgenic line. (B) Zn5 whole-mount immunostaining with Ath5::GAP43-RFP zebrafish transgenic line. Note that Zn5 overlaps with RFP-positive optic nerve and retinal progenitor cells (brackets) (C) GS and PCNA immunostainings on cryosections of Rx2::H2B-RFP medaka transgenic line. Rx2 dependent H2B-RFP expression clearly co-localizes with GS-positive Mueller glia cells (arrowheads) and PCNA positive retinal progenitor cells in the CMZ (brackets). Nuclei were counterstained with DAPI (blue).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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