ZFIN ID: ZDB-FIG-110526-18
Knight et al., 2011 - Ret signalling integrates a craniofacial muscle module during development. Development (Cambridge, England)   138(10):2015-2024 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-15 to Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage: Long-pec

Fig. 7 Gfra3 function is required for maintaining ret expression in opercular associated cells. (A-H) Expression of gfra3 (A,B), artn2 (C,D) and ret (E-H) in rethu2846 mutants (B,D) and wild-type (WT) siblings (A,C), stmhy024 mutants (F,H) and WT siblings (E,G) at 32 hours post-fertilisation (hpf; E,F) or 55 hpf (A-D,G,H). At 55 hpf, there are fewer gfra3-expressing cells in the forming adductor operculi (ao) and levator operculi (lo) (arrowheads) of rethu2846 mutants (B) compared with WT (A). By contrast, artn2 expression in the dorsal pharyngeal arches is unaffected in rethu2846 mutants (D) compared with WT (C). At 32 hpf, there are fewer ret+ cells (black arrowheads) in the dorsal second arch of stmhy024 mutants (F) relative to WT (E). Later, at 55 hpf ret expression in the opercular muscles is lost, but is unaffected in the anterior lateral line ganglia (white arrowhead, H). (I) A model for Ret signalling during opercular muscle development. Ret receptor and the Gfra3 co-receptor are present on myoblast cells (red). Artn2 from adjacent mesenchymal cells (blue) binds the Gfra3 co-receptor in Ret+ cells and muscle cells. Activation of Ret signalling by Gfra3 and Artn2 is required to maintain ret expression in myoblast cells and for myogenic differentiation to occur, involving myod expression and a loss of ret expression. Scale bars: 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
artnb WT standard conditions Long-pec hyoid muscle mesenchyme ISH
rethu2846/hu2846 standard conditions Long-pec hyoid muscle mesenchyme ISH
gfra3 WT standard conditions Long-pec hyoid muscle myoblast ISH
rethu2846/hu2846 standard conditions Long-pec hyoid muscle myoblast ISH
ret WT standard conditions Prim-15 hyoid muscle myoblast ISH
Long-pec hyoid muscle myoblast ISH
gfra3hh024/hh024 standard conditions Prim-15 anterior lateral line ganglion ISH
Prim-15 hyoid muscle myoblast ISH
Long-pec anterior lateral line ganglion ISH
Long-pec Not Detected hyoid muscle myoblast ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gfra3hh024/hh024 standard conditions Long-pec adductor operculi physical object quality, abnormal
Long-pec branchiomeric skeletal muscle development process quality, abnormal
Long-pec levator operculi physical object quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development