Fig. 2
Expression of Wnt pathway genes in zebrafish swimbladder as detected by WISH. (A–C) Expression of wnt5b in the mesenchyme of swimbladder at 36 hpf (A) and 72 hpf (B,C). (D–F) Expression of fz2 in the mesenchyme and outer mesothelium of swimbladder at 36 hpf (D) and 72 hpf (E,F). (G–I) Expression of fz7b in mesenchyme and outer mesothelium of swimbladder at 36 hpf (G) and 72 hpf (H,I). (J–L) Expression of lef1 in the mesenchyme and outer mesothelium of swimbladder at 36 hpf (J) and 72 hpf (K,L). (M–O) Expression of tcf3 in the epithelium and outer mesothelium of swimbladder at 36 hpf (M) to 72 hpf (N,O). Panels (C, F, I, L, O) are cross sections. All embryos were laterally oriented with anterior to the left unless specified. Dotted red circles indicated swimbladder and yellow circles indicated epithelium. Abbreviations: e, epithelium; g, gut; m, mesenchyme; n, notochord; o, outer mesothelium; pf, pectoral fin; pd, pneumatic duct; sb, swimbladder. Scale bars = 200 μm. Panel (A) scale bar applies to all whole mount images and Panel (C) scale bar is for all cross section images. |