FIGURE

Fig. 7

ID

ZDB-FIG-110516-12

Publication

Yin et al., 2011 - Wnt signaling is required for early development of zebrafish swimbladder

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Fig. 7

Effects of Wnt inhibition on cell proliferation in swimbladder.

hs:?Tcf-GFP fish was out-crossed with AB wild type fish, the resultant heterozygous embryos and their wild type siblings were heat-shocked at 66 hpf and fixed at 72 hpf for proliferation assay. (A?F) Proliferation assay detecting PCNA-positive cells (red) with DAPI counterstaining (green). The number of PCNA-positive cells (red) was greatly reduced in transgenic fishes (D?F) (n = 5) compared to that of controls (A?C) (n = 5). (G?L) Staining for phosphorylated histone H3 (PH3, red) with DAPI counterstaining (green). Compared to wild type fishes (G?I) (n = 5), the number of PH3-positive cells (red) was greatly reduced in transgenic fishes (D?F) (n = 5). Dotted white circles indicated swimbladder. Abbreviation: g, gut. Scale bar = 200 μm for all panels.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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