FIGURE

Fig. S2

ID

ZDB-FIG-110322-74

Publication

Burroughs-Garcia et al., 2011 - Evolutionarily conserved function of Gbx2 in anterior hindbrain development

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Fig. S2

Expression of GBX2ΔHB in injected embryos. A, B: Western blot of total lysates from 10 hpf embryos showing expression of mouse GBX2ΔHB protein translated from Gbx2ΔHB mRNA. Recombinant mouse V5- GBX2ΔHB fusion protein expressed in E. coli was used as a positive control. A1–3: Detection of GBX2ΔHB using chicken anti-GBX2 in (1) lysates from uninjected embryos, (2) lysates from embryos injected with 120 pg Gbx2ΔHB mRNA, (3) recombinant mouse V5- GBX2ΔHB fusion protein. A4: Detection of recombinant mouse V5-GBX2ΔHB fusion protein using anti-V5 antibody. B1–3: Detection of Myc-tagged GBX2ΔHB using anti-Myc (1) lysates from uninjected embryos, (2) lysates from embryos injected with 240 pg Gbx2ΔHB mRNA, (3) lysates from embryos injected with 120 pg Gbx2ΔHB mRNA. C: Tubulin loading control.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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