ZFIN Image: Burroughs-Garcia et al., 2011, Fig. S2

Image

Figure Caption

Fig. S2

Expression of GBX2ΔHB in injected embryos. A, B: Western blot of total lysates from 10 hpf embryos showing expression of mouse GBX2ΔHB protein translated from Gbx2ΔHB mRNA. Recombinant mouse V5- GBX2ΔHB fusion protein expressed in E. coli was used as a positive control. A1–3: Detection of GBX2ΔHB using chicken anti-GBX2 in (1) lysates from uninjected embryos, (2) lysates from embryos injected with 120 pg Gbx2ΔHB mRNA, (3) recombinant mouse V5- GBX2ΔHB fusion protein. A4: Detection of recombinant mouse V5-GBX2ΔHB fusion protein using anti-V5 antibody. B1–3: Detection of Myc-tagged GBX2ΔHB using anti-Myc (1) lysates from uninjected embryos, (2) lysates from embryos injected with 240 pg Gbx2ΔHB mRNA, (3) lysates from embryos injected with 120 pg Gbx2ΔHB mRNA. C: Tubulin loading control.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.