FIGURE

Fig. 5

ID

ZDB-FIG-110322-58

Publication

van der Velden et al., 2011 - The serine-threonine kinase LKB1 is essential for survival under energetic stress in zebrafish

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Fig. 5

Deregulation of PI3K signaling in lkb1 mutants. (A) Transverse sections of WT and lkb1-mutant livers at indicated days of development stained with an antibody against phospho-AKT. Strong phospho-AKT staining is detected in WT and lkb1-mutant livers at 5 dpf. WT liver is strongly stained at 7 dpf, whereas phospho-AKT staining is barely detectable in 7-dpf lkb1-mutant liver and in starved WT at 11 dpf. (B) Inhibition of PI3K signaling leads to a starvation-like phenotype in WT larvae. WT larvae at 7 dpf treated for 3 d with either LY29 or its inactive analog LY30. LY29 treatment of WT larvae at 4 dpf leads to dark liver (arrowheads) and abnormal hepatic steatosis as revealed by ORO staining. Treatment with the inactive analog LY30 has no effect in the morphology of the larvae.

Expression Data

Antibody:	Ab5-akt
Fish:	WT stk11^{hu1960/hu1960}
Condition:	fasting
Anatomical Term:	liver
Stage Range:	Day 5 to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	stk11^{hu1960/hu1960} stk11^{hu1968/hu1968}
Observed In:	regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA