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Fig. 5

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ZDB-FIG-110317-6

Publication

Gerety et al., 2011 - Morpholino artifacts provide pitfalls and reveal a novel role for pro-apoptotic genes in hindbrain boundary development

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Fig. 5

Pro-apoptotic Bcl genes puma and bax-a are required for normal boundary marker expression. Embryos injected with either of 2 different puma morpholinos (A–I) or a bax-a morpholino (JvL) show reduction or loss of rfng mRNA expression at the 21ss. Puma knockdown in wild-type (A–C) and tp53 mutant (D–I) embryos showed equivalent reductions in rfng expression (compare B, C and E, F, H, I). A krox20::GAL4 driver line was crossed to a UAS::puma line to force expression of Puma in rhombomeres 3 and 5. Double-positive embryos showed increased apoptosis (data not shown) and ectopic expression of rfng within these 2 segments (compare M and N, O). Similar ectopic rfng expression was detected when this GAL4 line was crossed to a UAS linked auto-activating Caspase3a (Rev-Casp3a, compare N, O and Q, R).

Expression Data

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Antibody Labeling

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Acknowledgments

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Reprinted from Developmental Biology, 350(2), Gerety, S.S., and Wilkinson, D.G., Morpholino artifacts provide pitfalls and reveal a novel role for pro-apoptotic genes in hindbrain boundary development, 279-289, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.