Fig. 2
- ID
- ZDB-FIG-110111-23
- Publication
- Tsujimura et al., 2010 - A single enhancer regulating the differential expression of duplicated red-sensitive opsin genes in zebrafish
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Regulatory ability of the 52 flanking regions of LWS-1 and LWS-2. (A) Schematic representation of the GFP or RFP expression constructs. (B) A transverse section of an adult Tg(LWS1up2.6kb:GFP-LWS2up1.8kb:RFP)#1631 retina. The dorsal side is oriented at the top of each panel and the ventral side is at the bottom. The left and middle panels show the GFP (green) and RFP (magenta) images, respectively. The right is the merge of the two panels with the DIC image. (C) A horizontal section of the retina from the same adult individual shown in (B). The nasal side is shown on the left and the temporal side is on the right. The upper and middle panels show the GFP (green) and RFP (magenta) images, respectively, and the lower one is the merge of the two panels with the DIC image. (D, E) Transverse (D) and horizontal (E) sections of an adult Tg(LWS1up2.6kb:GFP)#1509 retina. The left (D) and top (E) panels show images of GFP signals (green) and the right (D) and bottom (E) panels show their overlays with the DIC images. The dorsal side is oriented at the top of each panel and the ventral side is at the bottom in (D). The nasal side is oriented on the left and the temporal side is on the right in (E). (F) A vertical view of the photoreceptor layer magnified from the dashed rectangle in (D). The left panel shows the GFP signals (green) and the middle panel shows the signals of immunostaining against the zebrafish red opsin in the outer segments of LDCs (magenta). The right panel shows a merge of the left two panels. The overlap of the two signals appears as white. Scale bars = 100 μm for (B–E), 10 μm for (F). |
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| Stage: | Adult |