FIGURE

Fig. 7

ID

ZDB-FIG-101223-21

Publication

Santoriello et al., 2010 - Kita Driven Expression of Oncogenic HRAS Leads to Early Onset and Highly Penetrant Melanoma in Zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 7

Comparison between mitfa and kita Gal4 driver lines.

a) Schematic diagram of the construct and injection strategy used to transiently express GFP-HRASV12 in the mitfa:Gal4 and kita:Gal4 lines. b) Percentage of injected fish that display melanoytic hyperplasia/melanoma (MH) at 2 and 4 weeks (w) post fertilization. c–e) 4, 14 and 32dpf kita-Gal4 fish injected with UAS-GFP-HRASV12 plasmid for somatic integration. Mosaic expression of the transgene leads to abnormal melanocytes (c), persistent melanocytic hyperplasia (d) and melanoma (e) f–h) 4, 14 and 32dpf mitfa-Gal4 fish injected with UAS-GFP-HRASV12 plasmid. Mosaic expression of the transgene in this driver line generates fewer and smaller melanocytic lesions. Calibration bars = 2 mm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One