FIGURE

Fig. S1

ID

ZDB-FIG-101214-8

Publication

Kalousova et al., 2010 - Dachshund homologues play a conserved role in islet cell development

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Fig. S1

Effect of the dachb morpholino on the splicing pattern of the dachb mRNA and on the exocrine pancreas. A. Schematic representation of the first three exons of the dachb gene. Dachb morpholino targets the junction between exon 2 and intron 2–3.The arrows indicate the position of PCR primers relative to the exons, the introns, and the new splicing pattern. B. RT-PCR with RNA extracted from morpholino-injected embryos. Dachb morpholino induces a knock-down of the fully spliced dachb mRNA. The primers used are as indicated in (A).The presence of a portion on intron 2–3 is indicated by arrowheads. Lower panels: GFP reporter protein expression of Tg(ptfl a::GFP) embryos at 48 hpf in ptfl a::GFP embryos injected with the following morpholino oligonucleotides: standard control (C), mismatch control (D) and dachb morpholino (E). All panels are optical sections of ventrally viewed yolk-free embryos with anterior to the left. Scale bar, 50 μm.

Expression Data

Gene:	EGFP
Fish:	jh1Tg jh1Tg + MO2-dachb
Knockdown Reagent:	MO2-dachb
Anatomical Term:	exocrine pancreas
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	jh1Tg + MO2-dachb
Knockdown Reagent:	MO2-dachb
Observed In:	exocrine pancreas development
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 348(2), Kalousova, A., Mavropoulos, A., Adams, B.A., Nekrep, N., Li, Z., Krauss, S., Stainier, D.Y., and German, M.S., Dachshund homologues play a conserved role in islet cell development, 143-152, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.