FIGURE

Fig. 6

ID

ZDB-FIG-101206-22

Publication

den Hollander et al., 2010 - Genetic defects of GDF6 in the zebrafish out of sight mutant and in human eye developmental anomalies

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

Apoptosis in out^m233mutant embryos. (A - H) Transverse cryosections through the zebrafish eye. Apoptosis is detected using the TUNEL assay (red signal). Levels of apoptosis are abnormally high in out^m233mutant (E-H) eyes during early neurogenesis. At 30 hpf, there is an increase of apoptosis in the lens and the retina of mutants (E) compared to the wild-type (A). Apoptosis persists in and around the lens region at 48 hpf (F, compare to B). By 76 hpf, hardly any apoptosis is visible in the mutant eye (G), and a significantly increased amount of apoptosis occurs in the wild-type (C) retina, compared to the mutant. At 5 dpf, hardly any apoptotic cells are found in both the wild type (D) and the mutant (H). (I) The amount of cell death is quantitated. The average number of apoptotic cells per section is provided. Arrowheads point to the lens. n ≥ 9 sections from at least 3 embryos for each time point both for wild-type and mutant samples.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gdf6a^m233/m233 (AB)
Observed In:	apoptotic process
Stage Range:	Prim-15 to Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Genet.