FIGURE

Fig. 6

ID

ZDB-FIG-101122-67

Publication

North et al., 2010 - PGE2-regulated wnt signaling and N-acetylcysteine are synergistically hepatoprotective in zebrafish acetaminophen injury

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

Synergy between NAC and PGE2 limits toxicity and extends the therapeutic window in adult zebrafish after APAP. Adult zebrafish were exposed to APAP and NAC, dmPGE2, or a combination either concomitantly or with 18 h del. (A) GSH (nmoles/whole liver) was improved by combined exposure to NAC and dmPGE2. *ANOVA, n = 3?4, P < 0.01. (B) Relative caspase 3/7 levels were elevated after APAP and improved with immediate NAC and/or dmPGE2; delayed combinatorial therapy was still beneficial. *ANOVA, n = 4?5, P < 0.001. (C) Serum ALT levels (n = 10?20 fish/treatment at 24 hpe) show that combined NAC and dmPGE2 prevented hepatocyte damage by APAP, even after delay. (D) Combined NAC and dmPGE2 treatment led to improved adult zebrafish survival (n = 9?10/treatment) at 48 hpe. (E) Effect of delayed treatment on zebrafish liver histology at 24 hpe; sinusoidal hemorrhage and necrosis in APAP-treated adults could not be reversed with NAC or dmPGE2 alone but only with combined treatment.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA