Fig. S9

Gene structure and efficacies of larp7 and bcdin3 MOs. (A) Schematic representation of the D.rerio larp7 gene. Arrow represents the transcription start site. Exons are presented as black rectangles and introns as solid lines. The lower part of the schematic shows the enlarged region of the gene with exons 2 and 3 and the intron 2. The position of larp7 MO is indicated below the schematic and the primers used for assessing the MO efficacy are indicated above. (B) Efficacy of larp7 MO was determined by RT-PCR with the indicated primers in the schematic of A by using total RNA samples from control MO- or larp7 MO-injected zebrafish embryos. (C) Schematic representation of the D. rerio bcdin3 gene. Arrow represents the transcription start site. Exons are presented as black rectangles and introns as solid lines. The position of bcdin3 MO is indicated below the schematic and the primers used for assessing the MO efficacy are indicated above. (D) Efficacy of bcdin3 MO was determined by RT-PCR with the indicated primers in the schematic of panel C by using total RNA samples from control MO-or bcdin3 MO-injected zebrafish embryos.