ZFIN ID: ZDB-FIG-090911-3
Barboric et al., 2009 - 7SK snRNP/P-TEFb couples transcription elongation with alternative splicing and is essential for vertebrate development. Proceedings of the National Academy of Sciences of the United States of America   106(19):7798-7803 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 30%-epiboly to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 5 Knockdown of larp7 and bcdin3 in zebrafish caused developmental defects, decreased 7SK levels, and aberrant splicing. (A and B) RNA in situ hybridizations of whole-mount zebrafish embryos by using larp7 and bcdin3 antisense (1, 2) and sense probes (3, 4) at the indicated time points. Black arrowheads in panels 1 and 2 indicate larp7 and bcdin3 expressions in blastomeres and their enriched expressions in developing brain/head, respectively. (C) Zebrafish embryos injected with either larp7 MO or bcdin3 MO show developmental defects at 24 hpf compared with embryo injected with a control MO. Black arrowheads indicate the developing brain/head and trunk/tail. (D) Relative quantities of 7SK levels were determined by RT-qPCR at the indicated time points below the graph. The error bars represent the mean ± SD. (E) Splicing pattern analysis for the 2 genes indicated on the left at different time points as shown above the agarose gels. Levels of splicing variants were determined by RT-PCR by using the samples as in D and gene-specific primers.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
larp7 WT standard conditions 30%-epiboly blastoderm cell ISH
26+ somites brain ISH
matn3b WT standard conditions 10-13 somites whole organism RTPCR
Prim-5 whole organism RTPCR
Prim-25 whole organism RTPCR
Long-pec whole organism RTPCR
mepcea WT standard conditions 30%-epiboly blastoderm cell ISH
26+ somites brain ISH
neo1a WT standard conditions 10-13 somites whole organism RTPCR
Prim-5 whole organism RTPCR
Prim-25 whole organism RTPCR
Long-pec whole organism RTPCR
rn7sk WT standard conditions 10-13 somites whole organism RTPCR
Prim-5 whole organism RTPCR
Prim-25 whole organism RTPCR
Long-pec whole organism RTPCR
WT + MO1-larp7 standard conditions 10-13 somites whole organism RTPCR
Prim-5 whole organism RTPCR
Prim-25 whole organism RTPCR
Long-pec whole organism RTPCR
WT + MO1-mepcea standard conditions 10-13 somites whole organism RTPCR
Prim-5 whole organism RTPCR
Prim-25 whole organism RTPCR
Long-pec whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-larp7 standard conditions Prim-5 animal organ development disrupted, abnormal
Prim-5 brain degenerate, abnormal
Prim-5 embryo development disrupted, abnormal
WT + MO1-mepcea standard conditions Prim-5 animal organ development disrupted, abnormal
Prim-5 brain degenerate, abnormal
Prim-5 embryo development disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA