ZFIN ID: ZDB-FIG-101119-2
Gleason et al., 2009 - The transmembrane inner ear (Tmie) protein is essential for normal hearing and balance in the zebrafish. Proceedings of the National Academy of Sciences of the United States of America   106(50):21347-21352 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: Long-pec to Days 7-13
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: 26+ somites to Days 7-13

Fig. 2

Absence of gross morphological and developmental defects. In each panel, a WT larva (Upper) is contrasted with a mutant (Lower). (A, A′) Mutants display a normal body shape and length at 4 dpf. (B, B′) In a mutant, the semicircular canals develop normally and the anterior (AO) and posterior otoliths (PO) are normal. (C, C′) Labeling of hair cells by Tg(pou4f3:gap43-mGFP) (green) reveals no obvious differences in their number, shape, or localization in the anterior macula. (D, D′) In lateral-line neuromasts at 3 dpf, the hair bundles of mutants display normal planar cell polarity. The kinocilium of each hair bundle is marked by a black notch denoting the absence of actin staining by phalloidin. At 22 hpf (E, E′), 2 dpf (F, F′), and 3 dpf (G, G′), mutants display normal migration of the lateral-line primordium and formation of neuromasts, here labeled by Tg(cldnB:lynGFP) (green). OV, otic vesicle; arrowheads indicate the leading edges of the primordia. (H and I) Supporting cells labeled by Tg(cldnB:lynGFP) (green) are normally arranged in mutants in the anterior (H, H′) and posterior lateral lines (I, I′) of 5-dpf larvae. (J, J′) Fifty hours after treatment with Cu2+, a 7-dpf mutant larva shows normal regeneration of hair cells labeled by Et(krt4:GFP)sqet4 (green). (K, K′) Phalloidin staining shows that regenerated hair cells display planar cell polarity in a mutant. (Scale bars, 500 μm in A, F, and G; 50 μm in B; 5 μm in C; 2 μm in D and K; 100 μm in E; 10 μm in H–J.)

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP sqet4Et physical alteration: anatomical structure, chemical treatment: copper(II) sulfate Days 7-13 neuromast hair cell IFL
tmieru1000/ru1000; sqet4Et physical alteration: anatomical structure, chemical treatment: copper(II) sulfate Days 7-13 neuromast hair cell IFL
tmieru1000/ru1000; zf106Tg standard conditions Long-pec lateral line primordium IFL
Long-pec neuromast IFL
Protruding-mouth lateral line primordium IFL
Protruding-mouth neuromast IFL
Day 5 neuromast support cell IFL
zf106Tg standard conditions Long-pec lateral line primordium IFL
Long-pec neuromast IFL
Protruding-mouth lateral line primordium IFL
Protruding-mouth neuromast IFL
Day 5 neuromast support cell IFL
GFP s356tTg standard conditions Protruding-mouth hair cell anterior macula IFL
tmieru1000/ru1000; s356tTg standard conditions Protruding-mouth hair cell anterior macula IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
tmieru1000/ru1000 standard conditions Day 4 otolith physical object quality, normal
Day 4 semicircular canal physical object quality, normal
Day 4 whole organism length, normal
Day 4 whole organism shape, normal
tmieru1000/ru1000; s356tTg standard conditions Protruding-mouth hair cell anterior macula amount, normal
Protruding-mouth hair cell anterior macula position, normal
Protruding-mouth hair cell anterior macula shape, normal
tmieru1000/ru1000; sqet4Et physical alteration: anatomical structure, chemical treatment: copper(II) sulfate Days 7-13 neuromast hair cell physical object quality, normal
Days 7-13 regeneration occurrence, normal
tmieru1000/ru1000; zf106Tg standard conditions 26+ somites neuromast development process quality, normal
26+ somites neuromast primordium migration process quality, normal
Long-pec neuromast development process quality, normal
Long-pec neuromast primordium migration process quality, normal
Protruding-mouth neuromast development process quality, normal
Protruding-mouth neuromast primordium migration process quality, normal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA