FIGURE

Fig. 1

ID

ZDB-FIG-101119-1

Publication

Gleason et al., 2009 - The transmembrane inner ear (Tmie) protein is essential for normal hearing and balance in the zebrafish

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Fig. 1

Defective hair-cell function. (A) In a WT (Upper) and a mutant (Lower) larva at 6 dpf, arrowheads indicate neuromasts labeled by Tg(pou4f3:gap43-mGFP) (green). (B) Upon exposure to FM4?64 (red), the WT specimen (Upper) shows robust incorporation of the fluorophore into hair cells but the mutant (Lower) shows none. (C) In the inner ears of WT (Upper) and mutant (Lower) animals at 5 dpf, Tg(pou4f3:gap43-mGFP) (green) marks the anterior (AC), medial (MC), and posterior cristae (PC). (D) Pressure-injection of FM4?64 (red) into the otic cavities labels hair cells in the cristae of WT (Upper) but not mutant (Lower) fish. (E) Averaged extracellular recordings of microphonic potentials measured at 5?7 dpf from the inner ears of eight WT larvae show a clear response at twice the stimulus frequency, whereas the record from eight mutant larvae shows only a stimulus artifact. (Scale bars, 200 μm in A and B; 20 μm in C and D.)

Expression Data

Gene:	GFP
Fish:	s356tTg tmie^{ru1000/ru1000}; s356tTg
Anatomical Terms:	anterior crista lateral crista neuromast posterior crista
Stage Range:	Day 5 to Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tmie^{ru1000/ru1000} tmie^{ru1000/ru1000}; s356tTg
Observed In:	inner ear hair cell
Stage Range:	Day 5 to Days 7-13

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA