ZFIN ID: ZDB-FIG-101119-1
Gleason et al., 2009 - The transmembrane inner ear (Tmie) protein is essential for normal hearing and balance in the zebrafish. Proceedings of the National Academy of Sciences of the United States of America   106(50):21347-21352 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: Day 5 to Day 6
PHENOTYPE:
Fish:
Observed In:
Stage Range: Day 5 to Days 7-13

Fig. 1 Defective hair-cell function. (A) In a WT (Upper) and a mutant (Lower) larva at 6 dpf, arrowheads indicate neuromasts labeled by Tg(pou4f3:gap43-mGFP) (green). (B) Upon exposure to FM4–64 (red), the WT specimen (Upper) shows robust incorporation of the fluorophore into hair cells but the mutant (Lower) shows none. (C) In the inner ears of WT (Upper) and mutant (Lower) animals at 5 dpf, Tg(pou4f3:gap43-mGFP) (green) marks the anterior (AC), medial (MC), and posterior cristae (PC). (D) Pressure-injection of FM4–64 (red) into the otic cavities labels hair cells in the cristae of WT (Upper) but not mutant (Lower) fish. (E) Averaged extracellular recordings of microphonic potentials measured at 5–7 dpf from the inner ears of eight WT larvae show a clear response at twice the stimulus frequency, whereas the record from eight mutant larvae shows only a stimulus artifact. (Scale bars, 200 μm in A and B; 20 μm in C and D.)

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP s356tTg standard conditions Day 5 anterior crista IFL
Day 5 lateral crista IFL
Day 5 posterior crista IFL
Day 6 neuromast IFL
tmieru1000/ru1000; s356tTg standard conditions Day 5 anterior crista IFL
Day 5 lateral crista IFL
Day 5 posterior crista IFL
Day 6 neuromast IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
tmieru1000/ru1000 standard conditions Day 5 - Days 7-13 inner ear hair cell decreased functionality, abnormal
tmieru1000/ru1000; s356tTg standard conditions Day 5 inner ear hair cell decreased functionality, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA