Fig. S7

Regulation of PTCH1-cyclin B1 pathway by GRK2 is involved in gastrulation of zebrafish embryos. (A) Delayed gastrulation in GRK2 morphants which could be rescued by both zebrafish and bovine GRK2 catalytic domain. Each embryo was injected at the one- or two-cell stage with 2 ng con-MO, or 2 ng GRK2-MO alone or with 0.15 ng zebrafish or bovine GRK2 catalytic domain mRNA. Embryos were categorized, counted, and photographed at 6 hpf. The percentages of embryos in high-cell stage are shown. 200 embryos were counted for each bar. (B) GRK2, K220R, BP, or NLS-cyclin B1 rescues the arrest phenotype of GRK2 knockdown embryos. Embryos were injected with control morpholino (con-MO) or co-injected with 2 ng GRK2 morpholino (GRK2-MO) plus 0.15 ng wild type GRK2, one of the indicated GRK2 constructs, or 0.2 ng NLS-cyclin B1 mRNA. 200 embryos were counted for each bar. Percentage of arrest at 6 hpf was shown.