FIGURE

Fig. 4

ID

ZDB-FIG-101115-33

Publication

Padmanabhan et al., 2009 - Cardiac and vascular functions of the zebrafish orthologues of the type I neurofibromatosis gene NFI

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Fig. 4

MO knockdown of nf1a results in vascular patterning defects at 48 and 72 hpf. (A–C) At 48 hpf, nf1a ATG MO-treated Tg(fli:egfp)y1 (endothelial-specific GFP transgenic) zebrafish embryos display gross defects in vascular development compared with control MO-treated or uninjected samples. Morphant embryos (C) display abnormal claw-like projections at the leading edge of the developing intersomitic vessels and fail to develop the dorsal longitudinal anastomotic vessel (DLAV) present in both control MO-treated (B) and uninjected (A) samples. (D–F) At 72 hpf, nf1a ATG morphant embryos display only rudimentary DLAVs and a general disorganization of the trunk vasculature (F) when compared with control MO-treated (E) or uninjected (D) embryos. (Scale bars: 25 μm.)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA