FIGURE

Fig. S1

ID

ZDB-FIG-101115-28

Publication

Qin et al., 2009 - Genetic evidence for shared mechanisms of epimorphic regeneration in zebrafish

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Fig. S1

Isolation of GFP⁺ Müller glia. (A) Dissociated GFP⁺ Müller glial cell (green). Counterstained with DAPI (blue). (B and C) Flow cytometry scatter plots. forward scatter-height (FSC-H); side scatter-height (SSC-H). Dissociated cells from adult Tg(gfap:GFP)mi2002 zebrafish retinas were gated by forward and side scatters (B), and GFP⁺ Müller glia were isolated based on fluorescence in the FITC channel (R5) (C). Our yield of dissociated retinal cells from adult zebrafish (5- to 6-month old) was ~2.5 x 10⁵ cells/retina, of which ~9% were GFP⁺ Müller glia. With flow cytometry, we could recover ~2.1 x 10⁴ Müller glia/retina, representing an efficiency of ~84%. (Scale bar: 10 μm.)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA